~VHK antibodies from two rabbits recognized the same pattern of irnmunoreactive proteins in medic roots as a well-characterized antisenlm produced against purified SbPRP2 (Kleis-San Francisco and Tiemey, 1990), while antiserum from the third rabbit

During the period of DOE fundin~ we synthesized several PRP peptides,generated rabbit antisera against two PRP repeats found in early nodulin PRl%, and developed “confocal rnicros&py methods for ~oot irnmunohisto&ernistry. Using the anitbc@ies, we completed ext&&ke descriptive studies of PRP deposition in medic and alfalfa roots showing that PRPs deposition is developmentally regulated in roots and spatially restricted within the W* of specific root tissues. Domain-speaficantibodieswere isolated fiorn polyclorud sera using peptide affinity chromatography and were then used to demonstratethat nodukpdfic epitopesare shared by several nodubspecific proteins. The following provides a more detailed summary of this work